UNIVERSITY OF MICHIGAN MEDICAL SCHOOL

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Administrative Office
2570 MSRB II
1150 West Medical Center Drive
University of Michigan
Ann Arbor, MI 48109-0674
734 647 4776
734 936 2622 fax

Protein Structure Facility: Instrumentation

Peptide Synthesis

• Symphony Multiple Peptide Synthesizer (PTI, Inc); 2558 MSRB II; Peptide synthesis
• 433A Peptide Synthesizer with conductivity monitoring (Applied Biosystems); 2558 MSRB II; Peptide synthesis
• Two preparative HPLC instruments (Rainin, Beckman); 2558 MSRB II; Peptide purification
• Two analytical HPLC instruments (Rainin, Beckman); 2558 MSRB II; Peptide analysis

Mass Spectrometry

• MALDI micro TOF MXTM mass spectrometer (Waters Inc.); 2552 MSRB II; Protein and peptide analysis, peptide mass fingerprinting
  
  

• LCQ Duo Electrospray mass spectrometer (ThermoFinnigan); 2552 MSRB II; Peptide Quality Control

• Q-tof premier with nanoAcquity LC (Waters, Inc) ; 2552 MSRB II; LC-MS/MS analysis

• Waters UPLC-Qtof premier Instrument
  
  

  	Mass Accuracy	Peptides: +/-0.001% of Peptide MW (e.g. +/-0.2 Da for 2000 Da peptide) Proteins: +/-0.01% of Protein MW (e.g. +/-1.6 Da for 16kD protein)
  	Resolution:	10,000 (FWHM) with maximum sensitivity 15,000 - 20,000 (FWHM) with lower sensitivity
  	Practical Sensitivity:	(Intact Mw measurements): Peptides: 0.5 - 1 pmol/ul (inquire about lower concentrations) Proteins: 2-20pmol/ul depending on MW

Protein Analysis

• 494 HT Procise Protein Sequencer (Applied Biosystems); 2574 MSRB II; Edman Sequence analysis of proteins
• J 810 Sectropolarimeter (Jasco, Inc.); 2574 MSRB II, Circular Dichroism Analysis